Columbus, Ohio 28th March 2011 /Targetwire/ - Celartia, the pioneering Life Sciences group and manufacturers of PetakaTM reveal how some of the most innovative aspects of the product offers some truly unique applications in the research labs, cell line supply & cord blood collection .
Cells are normally cryopreserved for storage and transportation, and this process exposes cells to toxic chemicals such as DMSO. The patented gas diffusion channel in PetakaTM carefully regulates the cell and media exposure to oxygen thus maintaining a stable & optimum pH for cell culture. By simply withdrawing the cells from the heat source, they are put into a state of suspended animation induced by the moderate hypothermic temperatures which is characterized by cell cycle-G2 arrest, membrane changes, body shrinkage, spindle relaxation and metabolic changes. The result is a gene expression pattern adjustment, which is functional and entirely reversible when reintroducing the cells to heat at 37ºC.
In this state, cells may be left (depending on cell type) for between 2-8weeks without media change, allowing very simple, inexpensive short to medium term cell storage or pause of the cell culture, by simply maintaining the cells away from light and within a temperature range of between 16-22ºC. At these temperatures cells are arrested at G2/M phase. Those in G1 and S phase are not and pass through to G2 phase.
This application unique to PetakaTM offers a wide number of advantages other cell culture devices:
Take a look at some CHO cells at stages prior to dormancy, dormant state itself & then in recovery after dormancy by clicking here.Visit Celartia’s new comprehensive website at www.celartia.com
Celartia was founded in 2002 by Dr Emilio Barberá, having spent more than 40 years in cell and tissue culture, has developed a range of biotechnology products from mini-bioreactors to industrial bioreactors always following the philosophy of bringing new innovation to improve upon the long established inefficient protocols.
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